human thrombin antithrombin complex assaymax elisa kit  (Assaypro)

Journal: EJHaem

Article Title: SARS‐CoV‐2 infection results in upregulation of Plasminogen Activator Inhibitor‐1 and Neuroserpin in the lungs, and an increase in fibrinolysis inhibitors associated with disease severity

doi: 10.1002/jha2.654

Figure Lengend Snippet: Inhibitors and cofactors of the common and Protein C pathways in SARS‐CoV‐2 infection and controls. (A) Antithrombin, (B) TAT, Thrombin‐Antithrombin complex, (C) Heparin Cofactor II, (D) Protease Nexin‐1, (E) α2‐macroglobulin, (F) Protein Z, (G) Protein Z Inhibitor, (H) Protein C, (I) Protein S (total), and (J) Protein C Inhibitor plasma concentrations were determined by ELISA in controls and COVID‐19 patients. Each symbol corresponds to one patient. Means and SEM are indicated. NS, non‐significant; * p ≤ 0.05; **** p ≤ 0.0001 (Kruskal‐Wallis test, or Mann‐Whitney U test).

Article Snippet: ELISA for Antithrombin (AT, SERPINC1, EA3301‐1), Thrombin‐antithrombin complex (TAT; ET1020‐1), α‐2‐Antiplasmin (A2AP, SERPINF2, EA3477‐1), Plasmin‐α2‐Antiplasmin Complex (PAP; EP1807‐1), Tissue Plasminogen Activator/Plasminogen Activator Inhibitor‐1 complex (t‐PA/PAI‐1; EP1105‐1), Factor XIII (FXIII; EF1013‐1), α‐1‐Antitrypsin (A1AT, SERPINA1; EA5101‐1), Protein Z (PZ; EP3333‐1), Protein C (PC, EP2312‐1), Protein S (total) (PS, EP133‐1), and Vaspin (SERPIN12; EV3005‐1) from Assaypro, LLC (St. Charles, MO).

Techniques: Infection, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Circulatory Neutrophils Exhibit Enhanced Neutrophil Extracellular Trap Formation in Early Puerperium: NETs at the Nexus of Thrombosis and Immunity

doi: 10.3390/ijms222413646

Figure Lengend Snippet: Excessive NET formation by postpartum circulating neutrophils is complemented by activation of the coagulation cascade and alterations in the platelet activity status. ( a ) Immunohistochemical staining for TF (green), and DNA counterstain with DAPI (blue) after a 3 h in vitro culture. Magnification: 20×; Scale bars: 50 μm. ( b ) Platelet differential counts in healthy non-pregnant blood donors, donors during pregnancy and 48 h postpartum. ( c ) Comparative analysis between healthy donor controls, donors during gestation and postpartum, concerning the platelet morphology and activation indices mean platelet volume (MPV) and platelet distribution width (PDW). ( d ) TAT complexes concentration in citrate plasma from healthy donor controls, donors during pregnancy and postpartum, determined by ELISA. ( e ) Spearman correlation between TAT complexes concentration and NET-associated MPO/DNA complexes. ( f ) D-dimer levels in citrate plasma from healthy donor controls, donors during gestation and postpartum, determined by ELISA. P: pregnancy; PD: post-delivery. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one way ANOVA followed by Bonferroni’s multiple comparison post-test). All experiments were performed at least 6 times with consistent results.

Article Snippet: The concentrations of thrombin-antithrombin (TAT) complexes and D-dimers were measured by sandwich ELISA, utilizing the human TAT Complexes ELISA Kit (Assaypro, St. Charles, MO, USA) and the Imuclone D-Dimer ELISA Kit (American Diagnostica, Pfungstadt, Germany) respectively.

Techniques: Activation Assay, Coagulation, Activity Assay, Immunohistochemical staining, Staining, In Vitro, Concentration Assay, Enzyme-linked Immunosorbent Assay